Hotspot

trnH-GUG-psbA

LSC · 80-540 · 461 bp

Back to Gyrosphragma

Pi 0.0338
MarkerSeek score 66.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
461
variable_sites
13
pi
0.0338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 452-528 bp 1.0 84.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 466-542 bp 1.0 84.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 465-541 bp 1.0 84.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 467-543 bp 1.0 84.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 455-531 bp 1.0 84.5