Hotspot

atpF

LSC · 12522-13939 · 1418 bp

Back to Gyrinops

Pi 0.00289

MarkerSeek score 61.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1418
variable_sites: 11
pi: 0.00289
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCGTCAGCTCTAAGGGGTAC	ACAAAAAGGGACAGAGTTCT	55.0	40.0	58.2	54.4	1573-1578 bp	1.0	49.8
atpF_p2	TTTCGTCAGCTCTAAGGGGT	ACAAAAAGGGACAGAGTTCT	50.0	40.0	58.4	54.4	1575-1580 bp	1.0	48.8
atpF_p3	TCGTCAGCTCTAAGGGGTAC	ACAAAAAGGGACAGAGTTCTT	55.0	38.1	58.2	55.2	1573-1578 bp	1.0	48.7
atpF_p4	TTTCGTCAGCTCTAAGGGGT	ACAAAAAGGGACAGAGTTCTT	50.0	38.1	58.4	55.2	1575-1580 bp	1.0	47.7
atpF_p5	TTCGTCAGCTCTAAGGGGTA	ACAAAAAGGGACAGAGTTCT	50.0	40.0	57.5	54.4	1574-1579 bp	1.0	47.5