Hotspot

rpl32-trnL-UAG

IRa · 132580-133777 · 1198 bp

Back to Gyrinops

Pi 0.00921
MarkerSeek score 54.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
IRa
length_bp
1198
variable_sites
31
pi
0.00921
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGGTTTTCTTTCTACGGA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 52.3 57.9 1233-1286 bp 1.0 45.9
rpl32-trnL-UAG_p2 AAGGTTTTCTTTCTACGGAA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 52.3 57.9 1232-1285 bp 0.857143 39.0
rpl32-trnL-UAG_p3 AGGTTTTCTTTCTACGGAAA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 52.3 57.9 1231-1284 bp 0.857143 39.0
rpl32-trnL-UAG_p4 AAGGTTTTCTTTCTACGGAAA TCCTAAGAGCAGCGTGTCTA 33.3 50.0 53.3 57.9 1232-1285 bp 0.857143 39.0
rpl32-trnL-UAG_p5 AGGTTTTCTTTCTACGGAAAA TCCTAAGAGCAGCGTGTCTA 33.3 50.0 53.3 57.9 1231-1284 bp 0.857143 39.0