Hotspot

ndhF-rpl32

SSC · 131568-132372 · 805 bp

Back to Gyrinops

Pi 0.0207
MarkerSeek score 64.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
805
variable_sites
41
pi
0.0207
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGGTACGATCCATGAATATT TGCTCTAGAAGTACGCTTTT 33.3 40.0 52.1 54.4 868-898 bp 1.0 44.9
ndhF-rpl32_p2 AGGTACGATCCATGAATATTT TGCTCTAGAAGTACGCTTTT 33.3 40.0 52.1 54.4 867-897 bp 1.0 44.9
ndhF-rpl32_p3 AGGTACGATCCATGAATATTT TTGCTCTAGAAGTACGCTTT 33.3 40.0 52.1 54.4 868-898 bp 1.0 44.9
ndhF-rpl32_p4 AGGTACGATCCATGAATATTT TTTGCTCTAGAAGTACGCTT 33.3 40.0 52.1 54.4 869-899 bp 1.0 44.9
ndhF-rpl32_p5 AGGTACGATCCATGAATATTT TTTTGCTCTAGAAGTACGCT 33.3 40.0 52.1 54.4 870-900 bp 1.0 44.9