Hotspot

trnN-GUU-ycf1

IRb · 112370-112767 · 398 bp

Back to Gyrinops

Pi 0.00622

MarkerSeek score 61.40

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 398
variable_sites: 7
pi: 0.00622
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	ACGACCAGTCAGTTAACAGC	CCCAACGACCGAATTGCTTA	50.0	50.0	57.8	58.6	532-538 bp	1.0	76.3
trnN-GUU-ycf1_p2	AGGATTCGAACCTACGACCA	CCCAACGACCGAATTGCTTA	50.0	50.0	58.2	58.6	545-551 bp	1.0	76.2
trnN-GUU-ycf1_p3	GGATTCGAACCTACGACCAG	CCCAACGACCGAATTGCTTA	55.0	50.0	57.8	58.6	544-550 bp	1.0	75.8
trnN-GUU-ycf1_p4	ACCACTGAGCTACTGAGGAA	CCCAACGACCGAATTGCTTA	50.0	50.0	57.7	58.6	502-508 bp	1.0	75.1
trnN-GUU-ycf1_p5	CCCCAAGTAGGATTCGAACC	CCCAACGACCGAATTGCTTA	55.0	50.0	57.7	58.6	553-559 bp	1.0	74.8