Hotspot

trnF-GAA-ndhJ

LSC · 51508-52019 · 512 bp

Back to Gyrinops

Pi 0.0199

MarkerSeek score 69.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 23
pi: 0.0199
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CACGGCTGAAGCGTATCTTA	55.0	50.0	58.0	57.8	675-708 bp	1.0	82.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CACGGCTGAAGCGTATCTTA	50.0	50.0	58.1	57.8	639-672 bp	1.0	82.5
trnF-GAA-ndhJ_p3	TGGTAATGGTCGGGATAGCT	CACGGCTGAAGCGTATCTTA	50.0	50.0	57.9	57.8	686-719 bp	1.0	81.8
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAGAATCATCCACGGCTGAA	55.0	50.0	58.0	57.7	685-718 bp	1.0	81.7
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AGAATCATCCACGGCTGAAG	55.0	50.0	58.0	57.7	684-717 bp	1.0	81.7