Hotspot

petN-psbM

LSC · 30373-31473 · 1101 bp

Back to Gyrinops

Pi 0.00774

MarkerSeek score 62.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1101
variable_sites: 26
pi: 0.00774
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1209-1268 bp	1.0	82.2
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1210-1269 bp	1.0	81.2
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.6	58.3	1148-1207 bp	1.0	80.9
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	50.0	45.0	58.2	58.5	1208-1267 bp	1.0	80.4
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.6	57.6	1149-1208 bp	1.0	79.9