Hotspot

trnS-GCU-trnS-CGA

LSC · 8745-9572 · 828 bp

Back to Gyrinops

Pi 0.0101

MarkerSeek score 61.90

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 28
pi: 0.0101
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	CCCTTAACTACTGAGGGGGT	50.0	55.0	58.0	57.7	924-939 bp	1.0	80.9
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	CCCTTAACTACTGAGGGGGT	50.0	55.0	58.0	57.7	923-938 bp	1.0	80.9
trnS-GCU-trnS-CGA_p3	CGCACAACGGATTAGCAATC	CCCTTAACTACTGAGGGGGT	50.0	55.0	57.9	57.7	939-954 bp	1.0	80.2
trnS-GCU-trnS-CGA_p4	TCGCACAACGGATTAGCAAT	CCCTTAACTACTGAGGGGGT	45.0	55.0	58.3	57.7	940-955 bp	1.0	78.8
trnS-GCU-trnS-CGA_p5	AGCAATCCGACGCTTTAGTC	CCCTTAACTACTGAGGGGGT	50.0	55.0	58.4	57.7	926-941 bp	1.0	78.1