Hotspot

rps16-trnQ-UUG

LSC · 6212-7416 · 1205 bp

Back to Gyrinops

Pi 0.00712

MarkerSeek score 59.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1205
variable_sites: 23
pi: 0.00712
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1334-1485 bp	1.0	81.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.1	58.0	1293-1444 bp	1.0	81.8
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1345-1496 bp	1.0	81.2
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TTTGGTCCCGCTATTCGAAG	50.0	50.0	58.2	58.0	1304-1455 bp	1.0	81.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTTTTGGTCCCGCTATTCGA	45.0	50.0	58.1	58.3	1295-1446 bp	1.0	79.8