Hotspot

trnK-UUU-rps16

LSC · 4373-5072 · 700 bp

Back to Gyrinops

Pi 0.015
MarkerSeek score 55.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
700
variable_sites
26
pi
0.015
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AATTTGGTCCCTAGGGCTCT TCGAGACGTACGAGGAGAAA 50.0 50.0 58.0 57.9 1242-1248 bp 1.0 81.3
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TCGAGACGTACGAGGAGAAA 55.0 50.0 57.9 57.9 1076-1082 bp 1.0 80.9
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC TCGAGACGTACGAGGAGAAA 55.0 50.0 57.9 57.9 1075-1081 bp 1.0 80.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TCGAGACGTACGAGGAGAAA 50.0 50.0 58.2 57.9 1014-1020 bp 1.0 80.3
trnK-UUU-rps16_p5 AATTTGGTCCCTAGGGCTCT GCGGGGTTTTACGGAACTTA 50.0 50.0 58.0 58.2 982-987 bp 0.857143 74.5