Hotspot

ycf2

IRb · 85147-91557 · 6411 bp

Back to Gypsophila

Pi 0.000936

MarkerSeek score 53.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6411
variable_sites: 12
pi: 0.000936
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGTTTCTTTTGCCCCTTCCA	TGCCTCCATTTTGTTGTTGC	45.0	45.0	57.8	57.8	298 bp	1.0	78.6
ycf2_p2	TTTCTTTTGCCCCTTCCAGG	TGCCTCCATTTTGTTGTTGC	50.0	45.0	58.3	57.8	296 bp	1.0	77.7
ycf2_p3	CTTCCAGGCGATCGGAAAAT	TGCCTCCATTTTGTTGTTGC	50.0	45.0	58.3	57.8	284 bp	1.0	77.4
ycf2_p4	TGTTTCTTTTGCCCCTTCCA	GCCTCCATTTTGTTGTTGCT	45.0	45.0	57.8	57.5	297 bp	1.0	76.3
ycf2_p5	TGTTTCTTTTGCCCCTTCCA	TCCAAAGAATTCCCGCAAGA	45.0	45.0	57.8	57.4	246 bp	1.0	76.3