Hotspot

ycf1

SSC · 122852-128518 · 5667 bp

Back to Gypsophila

Pi 0.00206

MarkerSeek score 39.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5667
variable_sites: 23
pi: 0.00206
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTACGAATATACTTTCCCTAAA	GTAGCCGAACACACAAGGAA	30.4	50.0	52.2	58.1	245 bp	1.0	41.3
ycf1_p2	TCTACGAATATACTTTCCCTAAA	TCAGTAGCCGAACACACAAG	30.4	50.0	52.2	57.9	248 bp	1.0	41.3
ycf1_p3	TCTACGAATATACTTTCCCTAAA	TAGCCGAACACACAAGGAAG	30.4	50.0	52.2	57.8	244 bp	1.0	41.3
ycf1_p4	TCTACGAATATACTTTCCCTAAA	CCGAACACACAAGGAAGGAT	30.4	50.0	52.2	57.8	241 bp	1.0	41.3
ycf1_p5	TCTACGAATATACTTTCCCTAAAA	GTAGCCGAACACACAAGGAA	29.2	50.0	53.0	58.1	245 bp	1.0	41.3