Hotspot

rpoA

LSC · 77283-78314 · 1032 bp

Back to Gypsophila

Pi 0.00258

MarkerSeek score 60.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1032
variable_sites: 5
pi: 0.00258
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GCCCACCATGTGTATCTAGG	CCACATAATGGGTGTAGGCC	55.0	55.0	57.4	58.0	1247 bp	1.0	75.8
rpoA_p2	GCCCACCATGTGTATCTAGG	TGGGTGTAGGCCTCCTAAAA	55.0	50.0	57.4	58.0	1239 bp	1.0	75.5
rpoA_p3	GGGAATAGTCGCTTCAAGGT	CCACATAATGGGTGTAGGCC	50.0	55.0	57.3	58.0	1229 bp	1.0	74.6
rpoA_p4	GGGAATAGTCGCTTCAAGGT	TGGGTGTAGGCCTCCTAAAA	50.0	50.0	57.3	58.0	1221 bp	1.0	74.4
rpoA_p5	GCCCACCATGTGTATCTAGG	GGACGTAACTCCTATGCCAC	55.0	55.0	57.4	57.8	1263 bp	1.0	74.1