Hotspot

psbA

LSC · 321-1382 · 1062 bp

Back to Gypsophila

Pi 0

MarkerSeek score 31.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTCCGTTACTTTGACTTTCA	ACACGGGCATATAAGGCATG	40.9	50.0	58.2	58.1	1261-1262 bp	1.0	61.6
psbA_p2	TGCTCCGTTACTTTGACTTTCA	CACGGGCATATAAGGCATGT	40.9	50.0	58.2	58.1	1260-1261 bp	1.0	61.6
psbA_p3	TGCTCCGTTACTTTGACTTTCA	GACACGGGCATATAAGGCAT	40.9	50.0	58.2	57.8	1262-1263 bp	1.0	60.9
psbA_p4	GCTCCGTTACTTTGACTTTCA	ACACGGGCATATAAGGCATG	42.9	50.0	56.5	58.1	1260-1261 bp	1.0	59.1
psbA_p5	GCTCCGTTACTTTGACTTTCA	CACGGGCATATAAGGCATGT	42.9	50.0	56.5	58.1	1259-1260 bp	1.0	59.1