Hotspot

pafI

LSC · 42472-44569 · 2098 bp

Back to Gypsophila

Pi 0.00143

MarkerSeek score 55.80

Primer available yes

Label pafI

Feature Metadata

label_name: pafI
feature_type: gene
region: LSC
length_bp: 2098
variable_sites: 4
pi: 0.00143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI_p1	TCAGACAAACTAGAACAATCTGA	ATCCTTTTTCCACAAGCGGT	34.8	45.0	55.3	58.0	2251-2263 bp	1.0	40.5
pafI_p2	ACAAACTAGAACAATCTGATAAGGGA	ATCCTTTTTCCACAAGCGGT	34.6	45.0	58.1	58.0	2247-2259 bp	1.0	40.5
pafI_p3	AGTTCTTCCCATAAGAAAAATTCCA	ATCCTTTTTCCACAAGCGGT	32.0	45.0	56.9	58.0	2201-2213 bp	1.0	40.5
pafI_p4	ACAAACTAGAACAATCTGATAAGGG	ATCCTTTTTCCACAAGCGGT	36.0	45.0	56.8	58.0	2247-2259 bp	1.0	40.5
pafI_p5	CAGACAAACTAGAACAATCTGA	ATCCTTTTTCCACAAGCGGT	36.4	45.0	53.8	58.0	2250-2262 bp	1.0	40.5