Hotspot

ndhF

SSC · 109234-111486 · 2253 bp

Back to Gypsophila

Pi 0.00237

MarkerSeek score 44.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 10
pi: 0.00237
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAGTCCATTTTCTGGGGAA	AAATTGTCCGTAAAACAGGT	45.0	35.0	56.3	53.3	2714-2735 bp	1.0	40.9
ndhF_p2	TGAGTCCATTTTCTGGGGAA	AATTGTCCGTAAAACAGGTT	45.0	35.0	56.3	53.3	2713-2734 bp	1.0	40.9
ndhF_p3	TGAGTCCATTTTCTGGGGAA	AAATTGTCCGTAAAACAGGTT	45.0	33.3	56.3	54.2	2714-2735 bp	1.0	40.9
ndhF_p4	TGAGTCCATTTTCTGGGGAA	ACGAAAACCTCTTCATTAAGTGT	45.0	34.8	56.3	56.4	2883-2904 bp	1.0	40.8
ndhF_p5	TCCAGAAGAAGGACAAATTGGT	ACGAATACGCTTTTTGGAAA	40.9	35.0	57.5	54.1	2983-2999 bp	0.75	30.6