Hotspot

ndhD

SSC · 114630-116132 · 1503 bp

Back to Gypsophila

Pi 0.00266
MarkerSeek score 45.60
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
8
pi
0.00266
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 CACAAACATCAAGCATGCCC TTTTGGCCCAAGTGTATCTT 50.0 40.0 58.3 55.1 1616 bp 1.0 55.1
ndhD_p2 CACAAACATCAAGCATGCCC TTTTTGGCCCAAGTGTATCT 50.0 40.0 58.3 55.1 1617 bp 1.0 55.1
ndhD_p3 CACAAACATCAAGCATGCCC TTTTTGGCCCAAGTGTATCTT 50.0 38.1 58.3 55.9 1617 bp 1.0 53.7
ndhD_p4 CAAACATCAAGCATGCCCAA TTTTGGCCCAAGTGTATCTT 45.0 40.0 57.5 55.1 1614 bp 1.0 53.7
ndhD_p5 CAAACATCAAGCATGCCCAA TTTTTGGCCCAAGTGTATCT 45.0 40.0 57.5 55.1 1615 bp 1.0 53.7