Hotspot

accD

LSC · 56956-58386 · 1431 bp

Back to Gypsophila

Pi 0.00175

MarkerSeek score 60.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 5
pi: 0.00175
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCATGCAAATAGGGGGCAAA	TGGCCACAAAGAATTGACTT	45.0	40.0	57.8	55.7	1491 bp	1.0	60.2
accD_p2	TCATGCAAATAGGGGGCAAA	TTGGCCACAAAGAATTGACT	45.0	40.0	57.8	55.7	1492 bp	1.0	60.2
accD_p3	TTCATGCAAATAGGGGGCAA	TGGCCACAAAGAATTGACTT	45.0	40.0	57.8	55.7	1492 bp	1.0	60.2
accD_p4	TTCATGCAAATAGGGGGCAA	TTGGCCACAAAGAATTGACT	45.0	40.0	57.8	55.7	1493 bp	1.0	60.2
accD_p5	TTTCATGCAAATAGGGGGCA	TGGCCACAAAGAATTGACTT	45.0	40.0	57.8	55.7	1493 bp	1.0	60.2