Pi
0.000923
MarkerSeek score
60.10
Primer available
yes
Label
psbE-petL
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| psbE-petL_p1 | CAACCCGCAATGAATAGGGA | AGTCGTATCTTGTTCAGACCT | 50.0 | 42.9 | 57.9 | 56.1 | 1276-1284 bp | 1.0 | 57.2 |
| psbE-petL_p2 | AGAACGTTCTCCTGTGCTTC | AGTCGTATCTTGTTCAGACCT | 50.0 | 42.9 | 57.8 | 56.1 | 1199-1207 bp | 1.0 | 56.4 |
| psbE-petL_p3 | ACGAATAACCAACCCGCAAT | AGTCGTATCTTGTTCAGACCT | 45.0 | 42.9 | 58.2 | 56.1 | 1285-1293 bp | 1.0 | 56.3 |
| psbE-petL_p4 | CGAATAACCAACCCGCAATG | AGTCGTATCTTGTTCAGACCT | 50.0 | 42.9 | 57.8 | 56.1 | 1284-1292 bp | 1.0 | 56.1 |
| psbE-petL_p5 | GTTCTCCTGTGCTTCCAGAC | AGTCGTATCTTGTTCAGACCT | 55.0 | 42.9 | 58.2 | 56.1 | 1194-1202 bp | 1.0 | 56.1 |