Hotspot

petA-psbJ

LSC · 61977-62965 · 989 bp

Back to Gypsophila

Pi 0.0027

MarkerSeek score 63.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 5
pi: 0.0027
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTGCGTGTACAAGGTCT	TCTGGATTAGGCTCATCGCT	50.0	50.0	58.2	58.3	1109-1153 bp	1.0	78.4
petA-psbJ_p2	CCATTGCGTGTACAAGGTCT	TTCTGGATTAGGCTCATCGC	50.0	50.0	58.2	57.5	1110-1154 bp	1.0	76.5
petA-psbJ_p3	CCATTGCGTGTACAAGGTCT	ATTCTGGATTAGGCTCATCGC	50.0	47.6	58.2	57.9	1111-1155 bp	1.0	72.3
petA-psbJ_p4	CCATTGCGTGTACAAGGTCT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.2	58.1	1160-1204 bp	1.0	71.9
petA-psbJ_p5	CCATTGCGTGTACAAGGTCT	TCTGGATTAGGCTCATCGCTA	50.0	47.6	58.2	58.1	1109-1153 bp	1.0	71.7