Hotspot

ndhC-trnV-UAC

LSC · 50638-51598 · 961 bp

Back to Gypsophila

Pi 0.00104

MarkerSeek score 45.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 2
pi: 0.00104
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCTTTGCTAATCGGTGCTA	ACAAGCACAAGGGCTATAGC	50.0	50.0	57.7	58.0	1120 bp	1.0	77.2
ndhC-trnV-UAC_p2	CCCTTTGCTAATCGGTGCTA	GCACAAGGGCTATAGCTCAG	50.0	55.0	57.7	58.1	1116 bp	1.0	76.5
ndhC-trnV-UAC_p3	TCGGTGCTAAAATTCCGGAA	ACAAGCACAAGGGCTATAGC	45.0	50.0	57.5	58.0	1109 bp	1.0	76.0
ndhC-trnV-UAC_p4	CCCTTTGCTAATCGGTGCTA	GCACAAGCACAAGGGCTATA	50.0	50.0	57.7	58.2	1122 bp	1.0	75.5
ndhC-trnV-UAC_p5	TCGGTGCTAAAATTCCGGAA	GCACAAGGGCTATAGCTCAG	45.0	55.0	57.5	58.1	1105 bp	1.0	75.3