Hotspot

trnF-GAA-ndhJ

LSC · 48215-48980 · 766 bp

Back to Gypsophila

Pi 0.00327

MarkerSeek score 60.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 5
pi: 0.00327
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGGTAGGATG	55.0	55.0	58.0	58.0	912-921 bp	1.0	80.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGGTAGGATGG	55.0	55.0	58.0	58.0	911-920 bp	1.0	80.6
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATCCGCGCCTAAAACGTATT	55.0	45.0	58.0	58.1	938-947 bp	1.0	80.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGGTAGGATG	50.0	55.0	58.1	58.0	876-885 bp	1.0	80.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	875-884 bp	1.0	80.3