Hotspot

rps16-trnQ-UUG

LSC · 5899-6633 · 735 bp

Back to Gypsophila

Pi 0.00295
MarkerSeek score 63.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
735
variable_sites
4
pi
0.00295
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 825-851 bp 1.0 79.6
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.2 58.2 836-862 bp 1.0 79.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 826-852 bp 1.0 77.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTGGTAAGGCATCGGGTTTT 45.0 50.0 58.1 58.5 829-855 bp 1.0 77.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TGGTAAGGCATCGGGTTTTG 45.0 50.0 58.1 58.5 828-854 bp 1.0 77.3