Hotspot

rps4

LSC · 45323-45928 · 606 bp

Back to Gynura

Pi 0.00165
MarkerSeek score 59.70
Primer available yes
Label rps4

Feature Metadata

label_name
rps4
feature_type
gene
region
LSC
length_bp
606
variable_sites
1
pi
0.00165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4_p1 CGAAAGGCAGCAAAATGGG TCTGTAGAATGAAATTTCATCAA 52.6 26.1 57.9 52.1 739 bp 1.0 40.1
rps4_p2 CGAAAGGCAGCAAAATGGG TTCTGTAGAATGAAATTTCATCA 52.6 26.1 57.9 52.1 740 bp 1.0 40.1
rps4_p3 AAAGGCAGCAAAATGGGGT TCTGTAGAATGAAATTTCATCAA 47.4 26.1 58.1 52.1 737 bp 1.0 40.1
rps4_p4 AAAGGCAGCAAAATGGGGT TTCTGTAGAATGAAATTTCATCA 47.4 26.1 58.1 52.1 738 bp 1.0 40.1
rps4_p5 CGAAAGGCAGCAAAATGGG TTCTGTAGAATGAAATTTCATCAA 52.6 25.0 57.9 53.0 740 bp 1.0 40.1