Hotspot

rpoA

LSC · 76798-77805 · 1008 bp

Back to Gynura

Pi 0.00298
MarkerSeek score 60.50
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1008
variable_sites
3
pi
0.00298
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 TGAATCTATGCCGATTCCGAG CATAATGGCTGTAGACCCCC 47.6 55.0 57.7 57.4 1222 bp 1.0 65.2
rpoA_p2 GAATCTATGCCGATTCCGAGT CATAATGGCTGTAGACCCCC 47.6 55.0 57.7 57.4 1221 bp 1.0 65.2
rpoA_p3 TGAATCTATGCCGATTCCGA CATAATGGCTGTAGACCCCC 45.0 55.0 56.5 57.4 1222 bp 1.0 63.7
rpoA_p4 AATCTATGCCGATTCCGAGT CATAATGGCTGTAGACCCCC 45.0 55.0 56.5 57.4 1220 bp 1.0 63.6
rpoA_p5 TGAATCTATGCCGATTCCGAG ATAATGGCTGTAGACCCCCG 47.6 55.0 57.7 58.9 1221 bp 1.0 62.2