Hotspot

clpP

LSC · 68836-70857 · 2022 bp

Pi 0.00896

MarkerSeek score 63.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2022
variable_sites: 18
pi: 0.00896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGACGCGAAATACAGGCT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	2108-2111 bp	1.0	71.9
clpP_p2	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	2109-2112 bp	1.0	70.1
clpP_p3	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	58.0	56.6	2109-2112 bp	1.0	69.5
clpP_p4	CAAATCACGAATTGACGCGA	GGCTTTGTTACGTTTCCACA	45.0	45.0	57.5	56.9	2118-2121 bp	1.0	68.0
clpP_p5	TCAAATCACGAATTGACGCG	GGCTTTGTTACGTTTCCACA	45.0	45.0	57.5	56.9	2119-2122 bp	1.0	68.0