Hotspot

atpB

LSC · 52519-54015 · 1497 bp

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Pi 0.002
MarkerSeek score 59.90
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
LSC
length_bp
1497
variable_sites
3
pi
0.002
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 TCGATTAGGGGTCAGTACACA AAATCGTGTGCTAACTGCCA 47.6 45.0 57.9 58.1 1649 bp 1.0 70.6
atpB_p2 TCGATTAGGGGTCAGTACACA TCGTGTGCTAACTGCCATTT 47.6 45.0 57.9 58.1 1646 bp 1.0 70.6
atpB_p3 TCGATTAGGGGTCAGTACACA AATCGTGTGCTAACTGCCAT 47.6 45.0 57.9 57.9 1648 bp 1.0 70.5
atpB_p4 TCGATTAGGGGTCAGTACACA ATCGTGTGCTAACTGCCATT 47.6 45.0 57.9 57.9 1647 bp 1.0 70.5
atpB_p5 TCGATTAGGGGTCAGTACACA TCAACTTGCCATCGGACATT 47.6 45.0 57.9 57.8 1609 bp 1.0 69.8