Hotspot

ndhA-ndhI

SSC · 116373-116450 · 78 bp

Back to Gynura

Pi 0.0128
MarkerSeek score 59.90
Primer available yes
Label ndhA-ndhI

Feature Metadata

label_name
ndhA-ndhI
feature_type
igs
region
SSC
length_bp
78
variable_sites
1
pi
0.0128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA-ndhI_p1 AGATGGACTTTACCGAGGCT TGACCAATATACCTTGCGGC 50.0 50.0 58.1 58.0 269 bp 1.0 78.9
ndhA-ndhI_p2 AGATGGACTTTACCGAGGCT CAATATACCTTGCGGCTCGT 50.0 50.0 58.1 58.1 265 bp 1.0 78.2
ndhA-ndhI_p3 AGATGGACTTTACCGAGGCT ACCAATATACCTTGCGGCTC 50.0 50.0 58.1 57.7 267 bp 1.0 77.2
ndhA-ndhI_p4 AGATGGACTTTACCGAGGCT GACCAATATACCTTGCGGCT 50.0 50.0 58.1 57.7 268 bp 1.0 77.2
ndhA-ndhI_p5 AGATGGACTTTACCGAGGCT TATACCTTGCGGCTCGTACT 50.0 50.0 58.1 58.3 262 bp 1.0 76.7