Hotspot

psaI-ycf4

LSC · 58802-59193 · 392 bp

Back to Gynura

Pi 0.00765
MarkerSeek score 58.80
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
392
variable_sites
3
pi
0.00765
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GCAATCGCAATGGCTTCTTT TACTTGTTTTTCGAGCCCCC 45.0 50.0 58.0 58.1 507 bp 1.0 79.7
psaI-ycf4_p2 GCAATCGCAATGGCTTCTTT GGATAACAGCCCAGCAGAAA 45.0 50.0 58.0 57.9 528 bp 1.0 79.4
psaI-ycf4_p3 GCAATCGCAATGGCTTCTTT AAAGGATAACAGCCCAGCAG 45.0 50.0 58.0 57.9 531 bp 1.0 79.4
psaI-ycf4_p4 TAGGCCTAGTATTTCCGGCA TACTTGTTTTTCGAGCCCCC 50.0 50.0 57.9 58.1 524 bp 1.0 78.9
psaI-ycf4_p5 TAGGCCTAGTATTTCCGGCA GGATAACAGCCCAGCAGAAA 50.0 50.0 57.9 57.9 545 bp 1.0 78.7