Hotspot

ndhC-trnV(TAC)

LSC · 50261-51017 · 757 bp

Back to Gynura

Pi 0.00528
MarkerSeek score 61.80
Primer available yes
Label ndhC-trnV(TAC)

Feature Metadata

label_name
ndhC-trnV(TAC)
feature_type
igs
region
LSC
length_bp
757
variable_sites
4
pi
0.00528
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV(TAC)_p1 CCCTTTGCTAATCGGAGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.1 57.8 901-926 bp 1.0 71.5
ndhC-trnV(TAC)_p2 TCGGAGCTAAAAATCCCGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 56.9 57.8 890-915 bp 1.0 70.3
ndhC-trnV(TAC)_p3 CCCTTTGCTAATCGGAGCTAA AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.8 57.8 901-926 bp 1.0 69.2
ndhC-trnV(TAC)_p4 CCCTTTGCTAATCGGAGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.1 57.5 900-925 bp 1.0 68.7
ndhC-trnV(TAC)_p5 GGCCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 57.9 50.0 58.3 57.8 903-928 bp 1.0 68.4