Hotspot

atpI-atpH

LSC · 25117-26227 · 1111 bp

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Pi 0
MarkerSeek score 59.70
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1111
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TTTTTGCAACTTTAGCCGCG ATAACGGAAGCGGCAGAAAT 45.0 45.0 58.5 58.0 1189-1199 bp 1.0 76.3
atpI-atpH_p2 TTTTTGCAACTTTAGCCGCG TACCTTGACCAACTCCAGGT 45.0 50.0 58.5 57.9 1245-1255 bp 1.0 75.7
atpI-atpH_p3 TTTTTGCAACTTTAGCCGCG AGCCAATCCAGCAGCAATAA 45.0 45.0 58.5 57.9 1205-1215 bp 1.0 75.4
atpI-atpH_p4 TCAGTGGTTCCTATCCCTGT ATAACGGAAGCGGCAGAAAT 50.0 45.0 57.4 58.0 1250-1260 bp 1.0 75.3
atpI-atpH_p5 TTTTTGCAACTTTAGCCGCG AATAACGGAAGCGGCAGAAA 45.0 45.0 58.5 58.2 1190-1200 bp 1.0 75.1