Hotspot

psbK-psbI

LSC · 7764-8183 · 420 bp

Back to Gynura

Pi 0.00238
MarkerSeek score 56.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
420
variable_sites
1
pi
0.00238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TCTTCACGTCCTGGATTACG 50.0 50.0 57.9 57.1 557 bp 1.0 72.2
psbK-psbI_p2 TATGCCAGTCATACCCCTGT TCTTCACGTCCTGGATTACG 50.0 50.0 57.8 57.1 597 bp 1.0 71.1
psbK-psbI_p3 TGGCAAGCTGCTGTAAGTTT TCTTCACGTCCTGGATTACG 45.0 50.0 58.3 57.1 554 bp 1.0 70.2
psbK-psbI_p4 TTGGCAAGCTGCTGTAAGTT TCTTCACGTCCTGGATTACG 45.0 50.0 58.3 57.1 555 bp 1.0 70.2
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT TCTTCACGTCCTGGATTACG 45.0 50.0 58.3 57.1 556 bp 1.0 70.2