Hotspot

ycf1

SSC · 107426-112432 · 5007 bp

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Pi 0.00226

MarkerSeek score 42.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5007
variable_sites: 29
pi: 0.00226
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGGTTGGACAAATTATTCACA	TTGTAGAGCTATTCTGCGCA	34.8	45.0	56.6	57.0	231-237 bp	1.0	43.7
ycf1_p2	ACAGGTTGGACAAATTATTCACA	AGTTGTAGAGCTATTCTGCGC	34.8	47.6	56.6	58.2	233-239 bp	1.0	43.7
ycf1_p3	ACAGGTTGGACAAATTATTCACA	TGTAGAGCTATTCTGCGCAT	34.8	45.0	56.6	56.8	230-236 bp	1.0	43.7
ycf1_p4	AGATCTACAGGTTGGACAAA	TTGTAGAGCTATTCTGCGCA	40.0	45.0	53.6	57.0	237-243 bp	1.0	43.6
ycf1_p5	AGATCTACAGGTTGGACAAA	AGTTGTAGAGCTATTCTGCGC	40.0	47.6	53.6	58.2	239-245 bp	1.0	43.6