Hotspot

rpoC2

LSC · 19051-23199 · 4149 bp

Back to Gynoxys

Pi 0.00111

MarkerSeek score 54.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4149
variable_sites: 13
pi: 0.00111
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCACTCGAATCGCTTCAAGA	TTGGCCAAAATACCTTGCCT	50.0	45.0	58.0	58.0	232 bp	1.0	80.8
rpoC2_p2	GGCGAAGAGGCGAAGAAATA	TTGGCCAAAATACCTTGCCT	50.0	45.0	58.1	58.0	263 bp	1.0	80.2
rpoC2_p3	CCACTCGAATCGCTTCAAGA	GCCAAAATACCTTGCCTCCT	50.0	50.0	58.0	58.1	229 bp	1.0	79.8
rpoC2_p4	GGCGAAGAGGCGAAGAAATA	GCCAAAATACCTTGCCTCCT	50.0	50.0	58.1	58.1	260 bp	1.0	79.2
rpoC2_p5	GCCATTCTCGAGGGGAATTC	TTGGCCAAAATACCTTGCCT	55.0	45.0	58.4	58.0	292 bp	1.0	77.4