Hotspot

ndhA

SSC · 114192-116339 · 2148 bp

Back to Gynoxys

Pi 0.00171

MarkerSeek score 59.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2148
variable_sites: 10
pi: 0.00171
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTCCCCCGGGGTTTATTAAT	TGAACTCCGTTACCATAGGGA	50.0	47.6	56.9	57.9	2369-2374 bp	1.0	63.2
ndhA_p2	GTCCCCCGGGGTTTATTAAT	ACTCCGTTACCATAGGGAACA	50.0	47.6	56.9	58.2	2366-2371 bp	1.0	62.6
ndhA_p3	GTCCCCCGGGGTTTATTAATT	TGAACTCCGTTACCATAGGGA	47.6	47.6	57.7	57.9	2369-2374 bp	1.0	61.1
ndhA_p4	GTCCCCCGGGGTTTATTAATT	ACTCCGTTACCATAGGGAACA	47.6	47.6	57.7	58.2	2366-2371 bp	1.0	60.5
ndhA_p5	CCCCGGGGTTTATTAATTTGC	TGAACTCCGTTACCATAGGGA	47.6	47.6	57.5	57.9	2366-2371 bp	1.0	59.9