Hotspot

trnR-TCT-trnG-TCC

LSC · 29887-30101 · 215 bp

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Pi 0.0112

MarkerSeek score 60.30

Primer available yes

Label trnR-TCT-trnG-TCC

Feature Metadata

label_name: trnR-TCT-trnG-TCC
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 8
pi: 0.0112
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-trnG-TCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	289-295 bp	1.0	68.0
trnR-TCT-trnG-TCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	292-298 bp	1.0	67.7
trnR-TCT-trnG-TCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	295-301 bp	1.0	67.2
trnR-TCT-trnG-TCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	298-304 bp	1.0	67.0
trnR-TCT-trnG-TCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	293-299 bp	1.0	65.5