Hotspot

trnK-UUU-rps16

LSC · 4405-5244 · 840 bp

Back to Gynoxys

Pi 0.0031

MarkerSeek score 63.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 9
pi: 0.0031
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	970-976 bp	1.0	77.8
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	969-975 bp	1.0	77.8
trnK-UUU-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1055-1061 bp	1.0	77.1
trnK-UUU-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1054-1060 bp	1.0	77.1
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	985-991 bp	1.0	77.0