Hotspot

ccsA-ndhD

SSC · 118454-118738 · 285 bp

Back to Gynostemma

Pi 0.0697
MarkerSeek score 69.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
285
variable_sites
68
pi
0.0697
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGTTCATTTACATTAAGATCTGA CAGTTGACAAGGTCGAAGCT 29.2 50.0 54.0 58.1 238-360 bp 0.916667 48.7
ccsA-ndhD_p2 TGGTTCATTTACATTAAGATCTGA TCAGTTGACAAGGTCGAAGC 29.2 50.0 54.0 58.1 239-361 bp 0.916667 48.7
ccsA-ndhD_p3 ATGGTTCATTTACATTAAGATCTGA CAGTTGACAAGGTCGAAGCT 28.0 50.0 54.6 58.1 239-361 bp 0.916667 48.7
ccsA-ndhD_p4 TGGTTCATTTACATTAAGATCTGAT CAGTTGACAAGGTCGAAGCT 28.0 50.0 54.6 58.1 238-360 bp 0.916667 48.7
ccsA-ndhD_p5 TGGTTCATTTACATTAAGATCTGAT TCAGTTGACAAGGTCGAAGC 28.0 50.0 54.6 58.1 239-361 bp 0.916667 48.7