Hotspot

psbH-petB

LSC · 77351-77477 · 127 bp

Back to Gynostemma

Pi 0.0236

MarkerSeek score 61.70

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 8
pi: 0.0236
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GAACTACGCCTTTGATGGGT	TGACCAACTTTTTCTCTGTCGT	50.0	40.9	57.9	58.1	286-295 bp	0.916667	63.7
psbH-petB_p2	AACTACGCCTTTGATGGGTG	TGACCAACTTTTTCTCTGTCGT	50.0	40.9	58.2	58.1	285-294 bp	0.916667	63.1
psbH-petB_p3	GTGTCGCGATGGCTCTATTT	TGACCAACTTTTTCTCTGTCGT	50.0	40.9	58.4	58.1	268-277 bp	0.916667	61.4
psbH-petB_p4	TGTCGCGATGGCTCTATTTG	TGACCAACTTTTTCTCTGTCGT	50.0	40.9	58.4	58.1	267-276 bp	0.916667	61.4
psbH-petB_p5	GAACTACGCCTTTGATGGGT	TGACCAACTTTTTCTCTGTCG	50.0	42.9	57.9	56.4	286-295 bp	0.916667	60.1