Hotspot

petA-psbJ

LSC · 65226-66286 · 1061 bp

Back to Gynostemma

Pi 0.0344

MarkerSeek score 78.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 161
pi: 0.0344
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1775-1818 bp	1.0	91.6
petA-psbJ_p2	GGGCGTCAAGTGGTTGATAT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1880-1923 bp	1.0	91.4
petA-psbJ_p3	GGGCGTCAAGTGGTTGATAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1879-1922 bp	1.0	91.4
petA-psbJ_p4	GGGCGTCAAGTGGTTGATAT	ACGATGTTTTTGGAAGCCCT	50.0	45.0	58.0	58.0	1947-1990 bp	1.0	91.0
petA-psbJ_p5	GGGCGTCAAGTGGTTGATAT	ATGTTTTTGGAAGCCCTCGT	50.0	45.0	58.0	58.0	1944-1987 bp	1.0	91.0