Hotspot

ndhC-trnV-UAC

LSC · 53014-53841 · 828 bp

Back to Gynostemma

Pi 0.0241

MarkerSeek score 68.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 64
pi: 0.0241
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	865-989 bp	1.0	85.1
ndhC-trnV-UAC_p2	AGAGGGGCTAAAACTCCAGA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	864-988 bp	1.0	82.3
ndhC-trnV-UAC_p3	AGAGGGGCTAAAACTCCAGA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	853-977 bp	1.0	81.3
ndhC-trnV-UAC_p4	AGAGGGGCTAAAACTCCAGA	AAGGTCTACGGTTCGAGTCC	50.0	55.0	57.7	58.8	863-987 bp	1.0	80.0
ndhC-trnV-UAC_p5	AGAGGGGCTAAAACTCCAGA	GAGAAGGTCTACGGTTCGAG	50.0	55.0	57.7	57.2	866-990 bp	1.0	80.0