Hotspot

psaA-ycf3

LSC · 43935-44693 · 759 bp

Back to Gynostemma

Pi 0.0228

MarkerSeek score 74.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 56
pi: 0.0228
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	857-963 bp	1.0	84.9
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	853-959 bp	1.0	83.2
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.5	58.3	855-961 bp	1.0	83.2
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GGTTGAAGATCACAAGGCGT	50.0	50.0	58.5	58.5	788-894 bp	1.0	81.9
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	CGGAGGCTTGGTTCAATCAA	50.0	50.0	58.5	58.5	872-978 bp	1.0	81.5