Hotspot

psbC-trnS-UGA

LSC · 37210-37440 · 231 bp

Back to Gynostemma

Pi 0.0475

MarkerSeek score 69.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 31
pi: 0.0475
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	ACCTGTTCTTTTCATGACTCCT	GGGTTCGAATCCCTCTCTCT	40.9	55.0	57.3	58.2	246-285 bp	1.0	68.6
psbC-trnS-UGA_p2	AACCTGTTCTTTTCATGACTCCT	GGGTTCGAATCCCTCTCTCT	39.1	55.0	57.9	58.2	247-286 bp	1.0	65.8
psbC-trnS-UGA_p3	CCTGTTCTTTTCATGACTCCTCT	GGGTTCGAATCCCTCTCTCT	43.5	55.0	58.1	58.2	245-284 bp	1.0	65.5
psbC-trnS-UGA_p4	TGATCGTGATTTTGAACCTGT	GGGTTCGAATCCCTCTCTCT	38.1	55.0	55.8	58.2	261-300 bp	1.0	64.8
psbC-trnS-UGA_p5	ACCTGTTCTTTTCATGACTCC	GGGTTCGAATCCCTCTCTCT	42.9	55.0	55.8	58.2	246-285 bp	1.0	64.6