Hotspot

trnT-GGU-psbD

LSC · 33216-34778 · 1563 bp

Back to Gynostemma

Pi 0.0122
MarkerSeek score 62.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1563
variable_sites
61
pi
0.0122
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1658-1702 bp 1.0 79.8
trnT-GGU-psbD_p2 GGCGTAAGTCATCGGTTCAA GTCCCTCCGTAACCAGTCAT 50.0 55.0 58.3 58.8 1659-1703 bp 1.0 77.4
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CCTCCGTAACCAGTCATCCA 50.0 55.0 58.3 58.8 1656-1700 bp 1.0 77.4
trnT-GGU-psbD_p4 GGCGTAAGTCATCGGTTCAA CTCCGTAACCAGTCATCCAT 50.0 50.0 58.3 56.7 1655-1699 bp 1.0 73.7
trnT-GGU-psbD_p5 GTTCAAATCCGATAAGGGGCT TCCCTCCGTAACCAGTCATC 47.6 55.0 58.1 58.5 1644-1688 bp 1.0 73.5