Hotspot

rpoB-trnC-GCA

LSC · 27477-28649 · 1173 bp

Back to Gynostemma

Pi 0.0342

MarkerSeek score 75.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 103
pi: 0.0342
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCTCATTTCCACCCCCAAG	AGGATTTGCAGTCCCCCG	55.0	61.1	57.9	59.3	1160-1271 bp	1.0	63.0
rpoB-trnC-GCA_p2	TCTCTCATTTCCACCCCCAA	AGGATTTGCAGTCCCCCG	50.0	61.1	58.2	59.3	1161-1272 bp	1.0	62.1
rpoB-trnC-GCA_p3	TTCTCTCATTTCCACCCCCA	AGGATTTGCAGTCCCCCG	50.0	61.1	58.2	59.3	1162-1273 bp	1.0	62.1
rpoB-trnC-GCA_p4	CTCTCATTTCCACCCCCAAG	ATTTGCAGTCCCCCGCCTTA	55.0	55.0	57.9	61.9	1157-1268 bp	1.0	58.8
rpoB-trnC-GCA_p5	TCTCTCATTTCCACCCCCAA	ATTTGCAGTCCCCCGCCTTA	50.0	55.0	58.2	61.9	1158-1269 bp	1.0	57.9