Hotspot

atpH-atpI

LSC · 14111-15255 · 1145 bp

Back to Gynostemma

Pi 0.0317

MarkerSeek score 71.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 133
pi: 0.0317
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GATCCCTTCTACAGCTTGGC	TGTTTCGAATTGGTCAGGGG	55.0	50.0	58.0	58.1	1281-1718 bp	1.0	89.5
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	ACCATTCCTACTGACGGTCA	55.0	50.0	58.0	58.1	1412-1849 bp	1.0	89.4
atpH-atpI_p3	GATCCCTTCTACAGCTTGGC	AGCAAAACTCAGATTGGCGA	55.0	45.0	58.0	58.1	1352-1789 bp	1.0	89.3
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	ACCATTCCTACTGACGGTCA	45.0	50.0	58.1	58.1	1325-1762 bp	1.0	89.2
atpH-atpI_p5	GATCCCTTCTACAGCTTGGC	GGTTTCCAAGTACATGCCCA	55.0	50.0	58.0	58.1	1505-1942 bp	1.0	88.9