Hotspot

trnR-UCU-atpA

LSC · 10269-10578 · 310 bp

Back to Gynostemma

Pi 0.0944

MarkerSeek score 78.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 310
variable_sites: 64
pi: 0.0944
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TTCAAATCCTGTTGGACGCA	TCCACCAAGATATTCACCGC	45.0	50.0	58.0	57.7	410-769 bp	1.0	89.2
trnR-UCU-atpA_p2	TTCAAATCCTGTTGGACGCA	CCACCAAGATATTCACCGCT	45.0	50.0	58.0	57.7	409-768 bp	1.0	89.1
trnR-UCU-atpA_p3	TTCAAATCCTGTTGGACGCA	TGAAGCAGAAGCCCTTTTGA	45.0	45.0	58.0	57.6	390-749 bp	1.0	88.9
trnR-UCU-atpA_p4	TTCAAATCCTGTTGGACGCA	ATTCACCGCTGAAGCAGAAG	45.0	50.0	58.0	58.6	399-758 bp	1.0	87.4
trnR-UCU-atpA_p5	GTTCAAATCCTGTTGGACGC	TCCACCAAGATATTCACCGC	50.0	50.0	57.7	57.7	411-770 bp	1.0	86.7