Hotspot

trnS-GCU-trnG-UCC

LSC · 8650-9272 · 623 bp

Back to Gynostemma

Pi 0.0306

MarkerSeek score 70.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 54
pi: 0.0306
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGGCGCTTTA	TACCACTAAACTATACCCGC	45.0	45.0	58.0	54.0	619-717 bp	1.0	58.0
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGGCGCTTTA	TTACCACTAAACTATACCCGC	45.0	42.9	58.0	54.9	620-718 bp	1.0	56.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	668-766 bp	1.0	56.7
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	669-767 bp	1.0	55.6
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGGCGCTTTA	TTTACCACTAAACTATACCCGC	45.0	40.9	58.0	55.6	621-719 bp	1.0	55.2