Hotspot

trnH-GUG-psbA

LSC · 104-495 · 392 bp

Back to Gynostemma

Pi 0.0951

MarkerSeek score 76.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 96
pi: 0.0951
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	306-494 bp	0.833333	83.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	320-508 bp	0.833333	83.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	319-507 bp	0.833333	83.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	321-509 bp	0.833333	83.7
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	335-523 bp	0.833333	80.9