Hotspot

rpoA

LSC · 77513-78526 · 1014 bp

Back to Gynochthodes

Pi 0.00197

MarkerSeek score 53.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GACTTTTCCCTAGATACGTCT	AAGGCGTGTGTAGAAATGCC	42.9	50.0	54.2	58.8	1208-1209 bp	1.0	40.2
rpoA_p2	GACTTTTCCCTAGATACGTCT	AAAGGCGTGTGTAGAAATGC	42.9	45.0	54.2	57.0	1209-1210 bp	1.0	40.2
rpoA_p3	GACTTTTCCCTAGATACGTCT	GCGTGTGTAGAAATGCCGAT	42.9	50.0	54.2	59.0	1205-1206 bp	1.0	40.2
rpoA_p4	GACTTTTCCCTAGATACGTCT	CGTGTGTAGAAATGCCGATTG	42.9	47.6	54.2	58.0	1204-1205 bp	1.0	40.2
rpoA_p5	GACTTTTCCCTAGATACGTCT	AGGCGTGTGTAGAAATGCC	42.9	52.6	54.2	58.1	1207-1208 bp	1.0	40.2